Questions : If you have any questions, please contact our Scientific Support Team at 0 ext. They also offer maxi-prep of plasmids, with amounts up to 30 mg. Therefore, in order to receive a price quote, work order, and ETA specific for your project, please send all your project details to Services: Additional services offered by Bio Basic include codon optimization, for no additional charge. How to Receive a Price Quote: Bio Basic reviews all sequences before accepting a project. This can be supplied in any format, including WORD doc or SnapGene file. Final Expected Sequences of Plasmid(s), and relevant cloning instructions for BioBasic to follow (such as cloning sites to be used).
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When sending us instructions for your subcloning project, please provide: Our drivers take care of picking up the materials from your lab and shipping them to BioBasic for a fee of $40. Subcloning into one of the following vectors costs a standard subcloning price of $150:Ĭustom Vector Subcloning: Lastly, you can send your own vector to BioBasic, and they will perform the cloning for you (for the same fee of $150). Subcloning into one of the following vectors is also free of charge:īio Basic keeps many vectors in-house, shown below. Subcloning Services: The default is blunt end cloning into pUC57 with either Amp or Kan resistance. DNA cloning underlies all of biomedical research, yet cloning procedures often fail because they involve a large. All genes are verified by Sanger Sequencing, as well as other QC measures. What do I receive? Bio Basic supplies 4 μg of lyophilized plasmid containing your gene, and up to 10 μg for no extra charge. The proposed standard TAT gene synthesis is based on a gene with little to no complexities and with a length of about 1,000 bases. *Please note that the turnaround time is dependent on the complexity and length of a gene. Custom Genes:įrom the easiest to the most complicated gene syntheses, Bio Basic has the expertise. Our staff of experienced molecular biologists will be happy to assist you. For technical assistance contact us at call 0 ext.
![puc57 snapgene puc57 snapgene](https://media.addgene.org/data/easy-thumbnails/data/plasmids/140/140705/140705-map_w74CIsK5w7Zdw60cwoI.pdf.848x848_q85_autocrop.png)
We will send you a price quote and, upon your approval, procure and deliver your peptide. All peptides quality confirmed by Mass Spec and HPLCįill out this form to send us the details of the custom peptide you want.
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Finally, the construct was cloned in PUC57 in SnapGene 3.2.0.1 and PCR was simulated on hybrid vector using designed primers.įindings: Analysis confirmed that conserved regions for each gene were located on hybrid vector, and simulation of PCR proved the accuracy of designed primers.Ĭonclusion: A genetic simulator hybrid construct with the types of primers required to identify the four factors Francisla, Varivola, Borghuleria and Yersinia has been designed in silico so that such factors could be identified under positive control in emergencies.Custom Peptides & Genes from Canada-based Bio-Basic Inc. Specific primers were designed for each region using Oligo7, BioEdit, OligoAnalyzer tool as well as NCBI database. The sequence of these genes were obtained from NCBI in FASTA format and were aligned in BioEdit 7.0.5.3 software for finding conserve region of each gene, then some purposeful changes were applied in the sequence of each gene and the sequences were placed next to each other and the construct was designed. Materials & Methods: In this study, fopA, caf1, 16srRNA and HA genes were chosen to be located on the vector, to respectively represent Francisella, Yersinia, Burkholderia and Variolla. In this research we designed an in silico hybrid vector and relevant primers for detection of Francisella, Variola, Burkholderia and Yersinia. We can design specific primers for each region and use the hybrid vector as positive control sample in PCR. The idea of using hybrid vectors, containing genes of different pathogens, can overcome this limitation. Dehghan Esmatabadi 2, M Zeinoddini 2, N Pourmahdi 2ġ- Faculty of Chemistry & Chemical Engineering, Malek Ashtar University of Technology, Iran, Faculty of Chemistry & Chemical Engineering, Malek Ashtar University of Technology, IranĪims: Today, one of the most important problems in detection of human pathogens, is lack of positive control. In Silico Design of a Hybrid Structure as Positive Control for Simultaneous Detection of 4 Pathogenic Agents by PCR Method